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215391 Mouse Anti-Neurofilament Light (NF-L, NF68, NEFL, Neurofilament Light Polypeptide, NFL)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Porcine
Swiss Prot
P02547, P07196
Isotype
IgG1
Clone Number
DA2
Grade
Purified
Applications
IC IHC WB
Crossreactivity
Bo Ch Fe Hu Mo Po Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Neurofilaments are composed of three intermediate filament proteins: light (~68kD), medium (~160kD) and heavy (~200kD), which are involved in the maintenance of the neuronal caliber. Neurofilament light (NF68 or NF-L) is the most abundant of the three proteins.

Applications
Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:10,000-1:20,000 Immunohistochemistry: 1:100-1:500 Immunocytochemistry: 1:100-1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
A preparation of enzymatically dephosphorylated pig neurofilaments including NF-L, NF-M and NF-H.
Form
Supplied as a lyophilized powder in 5% trehalose. Reconstitute with sterile ddH2O.
Purity
Purified
Specificity
Recognizes porcine Neurofilament Light. Species Crossreactivity: Human, rat, mouse, feline, bovine, chicken
References
1. Felitsyn N. et al (2008) The heme precursor delta-aminolevulinate blocks peripheral myelin formation. J Neurochem. 2008 Sep;106(5):2068-79.
USBio References
No references available
Conjugates
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