Suitable for use in FLISA, Western Blot, Immunofluorescence, Immunoprecipitation and Flow Cytometry. Other applications not tested.

Immunohistochemistry: frozen Optimal working dilutions to be determined by researcher.

X63/Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Purified human b1 integrin preparation from HT1080 fibrosarcoma cell extract.

Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).

Purified by Protein G affinity chromatography from tissue culture supernatant.

Recognizes human beta1 integrin. Has been shown to bind to a ligand induced binding site antibody, binding being increased in the presence of fibronectin ligand. This antibody also enhances alpha 5-beta 1-fibronectin interactions. No crossreactivity with mouse or rat. Species Crossreactivity: mink