CD29 is also known as beta1 integrin or VLA-4 subunit alpha. CD29 is a ~130kD under reducing, ~115kD under non-reducing conditions single pass type I transmembrane glycoprotein. CD29 acts as the common beta subunit of the heterodimeric very late antigens 1-6, complexing with CD49a-f respectively where it forms part of the receptors for laminin, collagen and fibronectin. the VLA heterodimers mediate cell-cell and cell-matrix interactions.

Suitable for use in FLISA, Western Blot, Immunofluorescence, Immunoprecipitation and Flow Cytometry. Other applications not tested.

Immunohistochemistry: frozen Optimal working dilutions to be determined by researcher.

X63/Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Purified human b1 integrin preparation from HT1080 fibrosarcoma cell extract.

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.

Purified by Protein G affinity chromatography from tissue culture supernatant.

Recognizes human beta1 integrin. Has been shown to bind to a ligand induced binding site antibody, binding being increased in the presence of fibronectin ligand. This antibody also enhances alpha 5-beta 1-fibronectin interactions. No crossreactivity with mouse or rat. Species Crossreactivity: mink