Applications
Suitable for use in Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended dilutions
Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin sections. Optimal dilutions to be determined by the researcher.
Recommended Secondary Reagents
I1904-65N: F(ab')2 rabbit anti-mouse IgG HRP conjugate
Recommended Negative Control
I1904-79D: Mouse IgG1 Negative Control
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Occular melanoma cell line V+B2.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the beta 1 subunit of human integrin (CD29), which is a transmembrane glycoprotein expressed by most leukocytes.