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C2381-10A-ML550 Mouse Anti-CD29 (Integrin beta 1) (MaxLight 550)

Specifications
Brand
MaxLight™
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P05556
Conjugate
MaxLight™550
Isotype
IgG1
Clone Number
3H1196 (4B7R)
Grade
Affinity Purified
Applications
FC IHC IP
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.

Applications
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 10ul labels 10e6 cells in 100ul. Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin sections. Optimal dilutions to be determined by the researcher.
Recommended Secondary Reagents
I1904-65N: F(ab')2 rabbit anti-mouse IgG HRP conjugate
Recommended Negative Control
I1904-79D: Mouse IgG1 Negative Control
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Occular melanoma cell line V+B2.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the beta 1 subunit of human integrin (CD29), which is a transmembrane glycoprotein expressed by most leukocytes.
Conjugates
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