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G8965-09-PE Chicken Anti-Green Fluorescent Protein (GFP) (PE)

Specifications
References
Clone Type
Polyclonal
Host
Chicken
Swiss Prot
P42212
Conjugate
PE
Isotype
IgY
Grade
Purified
Applications
IC IP WB
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

Detects a band of the proper molecular weight, 30kD, in lysates from E. coli expressing GFP, but not in E. coli that do not express or in lysates prepared from E. coli containing the GFP-expressing plasmid but not induced, detect GFP in lysates from COS or primary neurons or fibroblast cells transfected with plasmids directing expression GFP or a tau-GFP fusion protein. Did not detect any signal when 50ug of protein from a rat brain lysate (negative control) was fractionated on the SDS-PAGE.

Applications
Suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation. Other applications have not been tested.
Recommended Dilutions
Immunocytochemistry: Cells were fixed using 4% formaldehyde in PBS, pH 7.4 for 20 minutes at RT. The cells are permeabilized with 0.2% Triton X-100 and 2% serum (corresponding to the animal in which the secondary antibody was raised) in PBS for 20 minutes at RT. Cell cultures are then incubated with primary antisera in PBS containing 2% serum (as above), at 4°C, overnight. Has also been used successfully with the avidin/biotin/peroxidase detection system. Cultures were washed four times (10 minutes each) between steps, and reaction product is developed with a 0.05% diaminobenzidine/ 0.02% hydrogen peroxide solution on ice for 8-10 minutes. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to Green Fluorescent Protein (GFP) containing a 6-His tag.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity
Purified
Specificity
Recognizes Green Fluorescent Protein (GFP). Has been used to detect GFP in primary neurons and zebrafish fibroblasts. Reactive with GFP from both native and recombinant sources. Species Crossreactivity: vertebrates
References
1. Hallam, S.J., et al., Nature Neuroscience 5: 1137-1146 (2002). 2. Sippy, T., et al., Nature Neuroscience 10: 1031-1038 (2003). 3. Jakobsson, J., et al., European Journal of Neuroscience 19: 761-765 (2004). 4. Zhao, S., et al., European Journal of Neuroscience 19: 1133-1140 (2004). 5. Yawata, S., et al., Journal of Neuroscience 26: 3626-3633 (2006).
USBio References
No references available
Conjugates
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