Suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation. Other applications have not been tested.

Immunocytochemistry: Cells were fixed using 4% formaldehyde in PBS, pH 7.4 for 20 minutes at RT. The cells are permeabilized with 0.2% Triton X-100 and 2% serum (corresponding to the animal in which the secondary antibody was raised) in PBS for 20 minutes at RT. Cell cultures are then incubated with primary antisera in PBS containing 2% serum (as above), at 4°C, overnight. Has also been used successfully with the avidin/biotin/peroxidase detection system. Cultures were washed four times (10 minutes each) between steps, and reaction product is developed with a 0.05% diaminobenzidine/ 0.02% hydrogen peroxide solution on ice for 8-10 minutes. Optimal dilutions to be determined by the researcher.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Recombinant protein corresponding to Green Fluorescent Protein (GFP) containing a 6-His tag.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

Purified

Recognizes Green Fluorescent Protein (GFP). Has been used to detect GFP in primary neurons and zebrafish fibroblasts. Reactive with GFP from both native and recombinant sources. Species Crossreactivity: vertebrates