Suitable for use in Flow Cytometry. Other applications not tested.

Flow Cytometry: 10ul labels 1x10e6 cells. Validated on bEND.3 cells and PC-12 cells. Optimal dilutions to be determined by the researcher.

1. Cells may be Fc-blocked with 1ug of mouse IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction. 2. After blocking, 10ul of N1276-91C was added to 1-2.5x10e5 cells and incubated for 30 minutes at RT. 3. Unbound antibody was removed by washing the cells twice in Flow Cytometry Staining Buffer (Catalog #F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Mouse Lyse Buffer. 4. The cells were resuspended in Flow Cytometry Staining Buffer for analysis by Flow Cytometry. As a control for this analysis, cells in a separate tube should be treated with CFS-labeled goat IgG antibody.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Carboxyfluorescein conjugates are sensitive to light.

Recombinant protein corresponding to Phe22-Ala810 (Lys811Arg), Ser829-Asp854 from rat Neuropilin-1, extracellular domain expressed in NSO cells. Species Sequence Homology: mouse; 98%, human; 93%

Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Carboxyfluorescein.

Purified by affinity chromatography.

Recognizes rat Neuropilin-1.