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N2159-50-FITC Mouse Anti-Neurofilament M 160-200kD (NF-M) (FITC)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Rat
Conjugate
FITC
Isotype
IgG1
Clone Number
3G201
Grade
Affinity Purified
Applications
FLISA IC IF IHC WB
Crossreactivity
Ch Hu Mo Rb Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
BSA Free

Neurofilaments (NFs) are prominent components of large myelinated axons. Based on sequence homology and intron placement, the NF genes have been classified as type IV IFs along with alpha-internexin, which is also expressed in the nervous system. NFs may function as effector proteins subject to a complex regulatory cross-talk between neurons and myelinating Schwann cells or oligrodendrocytes. Mammalian neurofilaments are assembled from light (NF-L), mid-sized (NF-M), and heavy (NF-H) neurofilament proteins. NF-M is required if myelinated axons are to achieve maximal diameters and NF-M has been shown to regulate the level of NF-L. NF-M subunits mainly regulate axonal caliber by altering the level of NF-L and in turn the number of NFs in the axon. Results seem to explain as a perturbation of NF stoichiometry in which overexpression of NF-M leads to the formation of perikaryal NF inclusions in motor neurons and the depletion of NFs from axons. Overexpression of NF-M in combination with NF-L increased radial growth of axons, which suggests that NF-M or NF-H must coassemble into filaments with NF-L before they can affect the radial growth of axons. The immature form of NFs composed of NF-L and NF-M, might function in establishing the early neuronal phenotype and in maintaining neurite outgrowth.

Applications
Suitable for use in Immunofluorescence/Immunocytochemistry, FELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Formalin fixed and paraffin embedded tissues and frozen Sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Rat NF-M
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes rat NF-M. Species Crossreactivity: human, mouse, chicken and rabbit
References
1. Elder, G.A., et al., J. Cell Biol. 143(1): 195–205 (1998). 2. Elder, G.A., et al., J. Neurosci. Res. 57(1): 23–32 (1999).
USBio References
No references available
Conjugates
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