Neurofilaments are intermediate (10–12nm) filaments located specifically in neurons. There are three classes of neurofilaments: NF-L (68kD), NF-M (160kD), and NF-H (200kD). The neurofilaments are long helical proteins which polymerize to form a rigid cytoskeleton in the neuron. This polymerized network is composed of all three filaments, and the stoichiometry of association varies during development. Neurofilaments are posttranslationally modified both by phosphorylation and glycosylation. Like other intermediate filament proteins, phosphorylation likely mediates neurofilament dynamics. However, how O-linked N-acetylglucosamine residues function to modify neurofilament function remains unclear.

Suitable for use in FLISA, Immunoblotting, Immunoprecipitation, Immunohistochemistry. Other applications not tested.

FLISA: 0.1–0.5ug/ml Immunohistochemistry: 5–10ug/ml (paraffin-embedded or frozen sections) Immunoblotting: 0.5–1.0ug/ml Immunoprecipitation: 2–5ug Optimal dilutions to be determined by the researcher.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Rat neurofilaments

Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.

Purified by Protein A affinity chromatography.

Reacts with the 160 kD polypeptides of human neurofilament. It specifically recognizes a phosphateindependent epitope in the tail (carboxy) domain of NF-M of most vertebrates and invertebrates.||Species crossreactivity: Human