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022892 WST-8 CAS: 193149-74-5

Specifications
References
CAS Number
193149-74-5
Grade
Highly Purified
Molecular Formula
C20H13N6O11S2•Na
Molecular Weight
600.47
Shipping Temp
RT
Storage Temp
-20°C
5-(2,4-Disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazolium Inner Salt Sodium Salt

A highly sensitive tetrazolium reagent that produces a water-soluble formazan (orange color that absorbs at 460 nm) in NADH. It is newer generation formazan-based dyes which release the converted product into the medium in a soluble form allowing for a non-destructive determination of viability enabling the cells to be subject to further investigations.

Synonyms
5-(2,4-Disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazolium Inner Salt Sodium Salt
CAS Number
193149-74-5
Molecular Weight
600.47
Molecular Formula
C20H13N6O11S2•Na
Appearance
White to off-white solid
Purity
≥98%
Storage and Stability
Store at -20°C under inert atmosphere. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
References
1. Ukeda, H., Kawana, D., Maeda, S., et al. Spectrophotometric assay for superoxide dismutase based on the reduction of highly water-soluble tetrazolium salts by xanthine-xanthine oxidase. Bioscience, Biotechnology, and Biochemistry 63(3), 485-488 (1999).|2. Ishiyama, M., Miyazono, Y., Sasamoto, K., et al. A highly water-soluble disulfonated tetrazolium salt as a chromogenic indicator for NADH as well as cell viability. Talanta 44, 1299-1305 (1997).|3. Sheng, X. et al.: Shand. Yiy., 46, 20 (2006)|4. Tsukani, T. et al.: J. Microbiol. Meth., 75, 109 (2008)
USBio References
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