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T8488 [2-(Trimethylammonium)ethyl] Methanethiosulfonate Bromide (MTSET Bromide) CAS: 91774-25-3

Specifications
References
CAS Number
91774-25-3
Grade
Highly Purified
Molecular Formula
C6H16BrNO2S2
Molecular Weight
278.23
EU Commodity Code
38220090
Shipping Temp
RT
Storage Temp
-20°C
N,N,N-Trimethyl-2-[(methylsulfonyl)thio]-ethanaminium Bromide; MTSET;|(2-Mercaptoethyl)trimethylammonium Bromide Methanesulfonate; |Methanethiosulfonate Ethyltrimethylammonium Bromide

Reacts specifically and rapidly with thiols to form mixed disulfides. Used to probe the structures of the ACh receptor channel of the GABAA receptor channel and of lactose permease.

At pH 7.5 and room temperature, MTSEA hydrolyzes with a half-life of about 15 minutes, MTSET hydrolyzes with a half-life of 10 minutes, and MTSES hydrolyzes with a half-life of 20 minutes. Routinely, one can use 2.5mM MTSEA, 1mM MTSET, or 10mM MTSES, applied for 1 to 5 minutes. (MTSET is 2.5 times as reactive with small sulfhydryl compounds as is MTSEA, and 10 times as reactive as MTSES). (Stauffer and Karlin, 1994; Karlin, personal communication).
For application to Xenopus Oocytes, dissolve the compounds in 115mM NaCl, 2.5mM KCl, 1.8mM MgCl2, 10mM Hepes, pH 7.5 with NaOH. MTS reagents decompose in buffer very quickly.
•DMSO is a good solvent for the MTS reagents which are not water soluble (i.e. the non-charged MTS reagents).
Synonyms
N,N,N-Trimethyl-2-[(methylsulfonyl)thio]-ethanaminium Bromide; MTSET; (2-Mercaptoethyl)trimethylammonium Bromide Methanesulfonate; Methanethiosulfonate Ethyltrimethylammonium Bromide
CAS No
91774-25-3
Molecular Formula
C6H16BrNO2S2
Molecular Weight
278.23
Purity
≥95%
Appearance
White solid
Solubility
Methanol (Slightly), Water (Slightly)
Method for Determining Identity
Proton NMR Spectroscopic and Mass Spectrometric Analysis
TLC Conditions
C18; Water: Isppropanol: Acetic Acid=6:4:1; Visualized with AMCS and KMnO4; Single Spot, Rf=0.65
Melting Point
151-158°C
Bromide
As Reported
Elemental Analysis
Calculated: %C: 25.90, %H: 5.80, %N: 5.03
Storage and Stability
May be stored at RT for short-term only. Long-term storage is recommended at -20°C. Stable for 6 months after receipt under inert atmosphere.. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Toxicity and Hazards
 All products should be handled by qualified personnel only, trained in laboratory procedures.
References
Duhten, R.L., et al.: Biochemistry, 32, 3139 (1993), Yang, N. et al.: Neuron, 16, 113 (1996), Kuner, T. et al.: Neuron, 17, 343 (1996), Holmgren, M. et al: Neuropharmacology, 35, 797 (1996), Chahine, M. et al.: Biochemical & Biophysical Res. Commun., 233, 606 (1997), Ehrlich, B.E., et al.: J. Gen. Physiol., 109, 255 (1997), Rassendren, F., et al.: The EMBO Journal, 16, 3446 (1997), Vedantham, V. and Cannon, C.C.: J. Gen. Physiol., 111, 83 (1998), Lin, C.-W. and Tsung-Yu, C.: J. Gen. Physiol., 116, 535 (2000), Sullivan, D.A. and cohen, J.B.: J. B.C., 275, 17, 12651 (2000)
USBio References
No references available
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