Eukaryotic Translation Initiation Factor 4E Binding Protein 1, 4E-BP1, 4EBP1, BP-1, BP1, MGC4316, PHAS I, PHASI, Phosphorylated Heat and Acid Stable Protein Regulated by Insulin 1, eIF4E Binding Protein 1, eIF4EBP1
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 2.5-5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
A 14aa peptide located near human 4EBP1 at the carboxy-terminus.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human 4EBP1 (C-TERMINAL). Species Crossreactivity: mouse.
References
1. Hara, K. et al (1997) Regulation of eIF4E BP1 phosphorylation by mTOR*. J. Biol. Chem. 272: 2645726463. 2. Haghighat, A. et al. (1995) Repression of capdependent translation by 4Ebinding|protein 1: competition with p220 for binding to eukaryotic initiation factor4E. EMBO J. 14: 57015709.
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