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0014-07J Mouse Anti-14-3-3, gamma (Protein kinase C inhibitor protein 1, KCIP-1, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide, YWHAG)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2a
Clone Number
KC21
Grade
Ascites
Applications
WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Protein kinase C inhibitor protein 1, KCIP-1, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide, YWHAG

14-3-3 proteins are present as multigene families in most organisms. Signal-induced phosphorylation has the ability to change protein function. Sometimes, however, phosphorylation is not enough to change a protein's function. 14-3-3 proteins play an important role of bringing signal transduction to completion. They regulate many cellular processes that are important in cancer biology, such as apoptosis and cell-cycle checkpoints.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:3,000. Detects a band of ~ 33kD. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
N-terminal fragment of human 14-3-3 gamma where the N-terminal Val was acetylated
Form
Supplied as a liquid. May contain azide. See Certificate of Analysis.
Purity
Mouse ascites
Specificity
Recognizes human 14-3-3 gamma. Reacts with the naturally occurring human form of 14-3-3 gamma, where the N-terminal Met is removed, resulting in an acetylated N-terminal Val. Unprocessed (non-modified) 14-3-3 gamma is not recognized by this antibody.
References
1. Ferl, R.J. 14-3-3 proteins:regulation of signal-induced events. Physiol. Plant. 120(2):173-178, 2004. |2. Hermeking, H. The 14-3-3 cancer connection. Nature. 3:931-943, 2003. |3. Jin, Z., et al. Nicotine induces multi-site phosphorylation of Bad in association with suppression of apoptosis. J. Biol. Chem. 279(22):23837-23844, 2004. (Immunoprecipitation, western blot, human)
USBio References
No references available
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