78-5000pg/ml.

29pg/ml.

< 10%

< 12%

*026335A: Microtiter Plate, 1x96wells, Pre-coated, ready to use *026335B: Standard, 2x1vial 026335C: Standard Diluent, 1x20ml *026335D: Detection Reagent A, 1x120ul *026335E: Detection Reagent B, 1x120ul 026335F: Assay Diluent A, 1x12ml 026335G: Assay Diluent B, 1x12ml 026335H: TMB Substrate, 1x9ml 026335K: Stop Solution, 1x6ml 026335L: Wash Buffer, 30X, 1x20ml

The Stop Solution (026335K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Store *026335A, *026335B, *026335D and *026335E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

1. Prepare all reagents, samples and standards; 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C. 3. Aspirate and add 100ul prepared Detection Reagent A immediately. Incubate 1 hour at 37°C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C. 6. Aspirate and wash 5 times. 7. Add 90ul 026335H: TMB Substrate. Incubate 15-25 minutes at 37°C. 8. Add 50ul 026335K: Stop Solution. Read at 450nm immediately.