31.2-2000pg/ml

11.8pg/ml

<10%

<12%

*027053A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use *027053B: Standard, 2x1 vial 027053C: Standard Diluent, 1x20ml *027053D: Detection Reagent A (green), 1x120ul *027053E: Detection Reagent B (red), 1x120ul 027053F: Assay Diluent A, 1x12ml 027053G: Assay Diluent B, 1x12ml 027053H: TMB Substrate, 1x9ml *027053J: Positive Control, 104.4pg/ml 1x1 vial 027053K: Stop Solution, 1x6ml 027053L: Wash Buffer, 30X, 1x20ml

The Stop Solution (027053K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

The control vials are from human serum sample known to contain Ntn1. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

The control vial contain human Ntn1 in human serum (lyophilized powder). Note: The human based serum used in this product are screened for HIV, Hepatitis A, B, C, HTLV and Syphilis.

104.4pg/ml

Disslove with 150ul standard dilutent and let stand for 10 minutes. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: After reconstitution, store Positive Control vial at 4°C and use within 5 days.

Store *027053A, *027053B, *027053D, *027053E and *027053J at -20°C. Store all the other components at 4°C. After reconstitution of *027053J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C. 6. Aspirate and wash 5 times. 7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C. 8. Add 50ul Stop Solution. Read at 450nm immediately.

Human

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.