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029673 MYL5, Recombinant, Human, aa1-173, His-Tag (Myosin Light Chain 5, Myosin Regulatory Light Chain 5, Superfast Myosin Regulatory Light Chain 2, MYLC2, MyLC-2) CAS:

Specifications
References
Grade
Highly Purified
Accession Number
NP_002468
Molecular Weight
22.1
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

MYL5, is a hexameric ATPase cellular motor protein. Myosin is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL5 is a regulatory light chain and is expressed in fetal muscle and in adult retina, cerebellum, and basal ganglia. Reconstitution of myosin with regulatory light chain 5 or alkali light chain increases filament velocity to intermediate rates, and readdition of both classes of light chains fully restores the original sliding velocity.

Recombinant corresponding to aa1-173 from human MYL5, fused to His-tag at N-terminal expressed in E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMASRKT KKKEGGALRA QRASSNVFSN FEQTQIQEFK EAFTLMDQNR DGFIDKEDLK DTYASLGKTN VKDDELDAML KEASGPINFT MFLNLFGEKL SGTDAEETIL NAFKMLDPDG KGKINKEYIK RLLMSQADKM TAEEVDQMFQ FASIDVAGNL DYKALSYVIT HGEEKEE
Molecular Weight
~22.1kD (MALDI-TOF)
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
≥90% (SDS-PAGE)
Concentration
~0.5mg/ml
Form
Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 20% glycerol, 0.1M sodium chloride, 1mM DTT, 0.1mM PMSF.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Collins C., et al. (1993) Hum Mol Genet. 1:727-733. 2. Lowey S., et al. (1993) Nature. 365:454-456.
USBio References
No references available
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