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029676 NAPG, Recombinant, Human, aa1-312, His-Tag (Gamma-soluble NSF Attachment Protein, SNAP-gamma, N-ethylmaleimide-sensitive Factor Attachment Protein gamma, SNAPG) CAS:

Specifications
References
Grade
Highly Purified
Accession Number
NP_003817
Molecular Weight
37.3
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

NAPG, is a cytoplasmic protein that binds to a membrane receptor complex composed of VAMP, SNAP25 and syntaxin. It mediates the membrane binding of NSF, which is essential for membrane fusion reactions. An additional protein designated synaptophysin may regulate exocytosis by competing with SNAP25 and syntaxins for VAMP binding.

Source
Recombinant corresponding to aa1-312 from human NAPG, fused to His-tag at N-terminal expressed in E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAAQKI NEGLEHLAKA EKYLKTGFLK WKPDYDSAAS EYGKAAVAFK NAKQFEQAKD ACLREAVAHE NNRALFHAAK AYEQAGMMLK EMQKLPEAVQ LIEKASMMYL ENGTPDTAAM ALERAGKLIE NVDPEKAVQL YQQTANVFEN EERLRQAVEL LGKASRLLVR GRRFDEAALS IQKEKNIYKE IENYPTCYKK TIAQVLVHLH RNDYVAAERC VRESYSIPGF NGSEDCAALE QLLEGYDQQD QDQVSDVCNS PLFKYMDNDY AKLGLSLVVP GGGIKKKSPA TPQAKPDGVT ATAADEEEDE YSGGLC
Molecular Weight
~37.3kD (MALDI-TOF)
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
≥95% (SDS-PAGE)
Concentration
~1mg/ml
Form
Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 10% glycerol, 0.1M sodium chloride, 1mM DTT.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Bennett M.K. et al. (1993) Cell 74: 863-873. 2. McMahon H.T. et al. (1995) J. Biol. Chem. 270: 2213-2217.
USBio References
No references available
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