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031118 Mouse Anti-MED14 (MED14, ARC150, CRSP2, CXorf4, DRIP150, EXLM1, RGR1, TRAP170, Mediator of RNA polymerase II transcription subunit 14, Activator-recruited cofactor 150kD component, Cofactor required for Sp1 transcriptional activation subunit 2, Mediator complex subunit 14, RGR1 homolog, Thyroid hormone receptor-associated protein complex 170kD component, Transcriptional coactivator CRSP150, Vitamin D3 receptor-interacting protein complex 150kD component)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgM
Clone Number
483CT11.1.1
Grade
Ascites
Applications
E WB
Crossreactivity
Hu Mo
Accession #
NP_004220.2; O60244
Gene ID
9282
Gene #
MED14
Shipping Temp
Blue Ice
Storage Temp
-20°C
MED14, ARC150, CRSP2, CXorf4, DRIP150, EXLM1, RGR1, TRAP170, Mediator of RNA polymerase II transcription subunit 14, Activator-recruited cofactor 150kD component, Cofactor required for Sp1 transcriptional activation subunit 2, Mediator complex subunit 14, RGR1 homolog, Thyroid hormone receptor-associated protein complex 170kD component, Transcriptional coactivator CRSP150, Vitamin D3 receptor-interacting protein complex 150kD component

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation.

Applications
Suitable for use in Western Blot, ELISA.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:500~8000
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified His-tagged MED14 protein (fragment).
Form
Supplied as a liquid, 0.09% sodium azide.
Purity
Ascites
Specificity
Human, mouse
References
Wu, C., et al. Proteomics 7(11):1775-1785(2007)|Lee, J., et al. Arch. Biochem. Biophys. 461(2):200-210(2007)|Olsen, J.V., et al. Cell 127(3):635-648(2006)|Olsen, J.V., et al. Cell 127(3):635-648(2006)|Chen, W., et al. Mol. Endocrinol. 20(3):560-572(2006)
USBio References
No references available
Conjugates
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