Bckdk, ID

Clone Type

Polyclonal

Host

Rabbit

Source

Human

Isotype

IgG

Grade

Affinity Purified

Applications

E WB

Crossreactivity

Hu Mo

Accession #

NP_033869.1; O55028

Gene ID

12041

Gene #

Bckdk

Shipping Temp

Blue Ice

Storage Temp

-20°C

Bckdk, [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial, Branched-chain alpha-ketoacid dehydrogenase kinase

Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex (By similarity).

Applications

Suitable for use in Western Blot, ELISA

Recommended Dilution

ELISA: 1:1,000 Western Blot: 1:100-500

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Mouse Bckdk antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-188 amino acids from the Central region of Mouse Bckdk.

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Purity

Purified by Protein A affinity chromatography.

Specificity

Human, mouse

References

Pagliarini, D.J., et al. Cell 134(1):112-123(2008)|Lee, J., et al. Mol. Cell Proteomics 6(4):669-676(2007)|Hutson, S.M. Biochem. J. 400 (1), E1-E3 (2006) :|Joshi, M.A., et al. Biochem. J. 400(1):153-162(2006)|Trinidad, J.C., et al. Mol. Cell Proteomics 5(5):914-922(2006)

USBio References

No references available

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032453 Rabbit Anti-Bckdk, ID (Bckdk, [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial, Branched-chain alpha-ketoacid dehydrogenase kinase)

Clone Type

Host

Source

Isotype

Grade

Applications

Crossreactivity

Accession #

Gene ID

Gene #

Shipping Temp

Storage Temp

Bckdk, [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial, Branched-chain alpha-ketoacid dehydrogenase kinase

References

USBio References

Conjugates

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