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033473 Rabbit Anti-CD1E, ID (CD1E, T-cell surface glycoprotein CD1e, membrane-associated, R2G1, CD1e, T-cell surface glycoprotein CD1e, soluble)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P15812
Isotype
IgG
Grade
Affinity Purified
Applications
E FC IF IHC WB
Crossreactivity
Hu
Accession #
NP_112155.2
Gene ID
913
Gene #
CD1E
Shipping Temp
Blue Ice
Storage Temp
-20°C
CD1E, T-cell surface glycoprotein CD1e, membrane-associated, R2G1, CD1e, T-cell surface glycoprotein CD1e, soluble

CD1E encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes within Golgi compartments, endosomes, and lysosomes, and is cleaved into a stable soluble form. The soluble form is required for the intracellular processing of some glycolipids into a form that can be presented by other CD1 family members.

Applications
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Indirect Flow Cytometry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Indirect Flow Cytometry: 1:10-50 Immunofluorescence: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
CD1E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-304 amino acids from the Central region of human CD1E.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human
References
Maitre, B., et al. Biochem. J. 419(3):661-668(2009)|Kuijf, M.L., et al. J. Neuroimmunol. 205 (1-2), 110-112 (2008) |Maitre, B., et al. Traffic 9(4):431-445(2008)
USBio References
No references available
Conjugates
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