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034559 Rabbit Anti-DDX53, CT (DDX53, CAGE, Probable ATP-dependent RNA helicase DDX53, Cancer-associated gene protein, Cancer/testis antigen 26, DEAD box protein 53, DEAD box protein CAGE)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
Q86TM3
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_874358.2
Gene ID
168400
Gene #
DDX53
Shipping Temp
Blue Ice
Storage Temp
-20°C
DDX53, CAGE, Probable ATP-dependent RNA helicase DDX53, Cancer-associated gene protein, Cancer/testis antigen 26, DEAD box protein 53, DEAD box protein CAGE

DDX53 belongs to the DEAD box helicase family. It is expressed in testis and shows wide expression in various cancer tissues and cancer cell lines.

Applications
Suitable for use in Western Blot and ELISA. Other applications have not been tested.
Recommended Dilution
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 590-619 in the C-terminal region of human DDX53, UniProt Accession #Q86TM3.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human DDX53.
References
1. Kim, Y., et al. J. Biol. Chem. 285(34):25957-25968(2010) 2. Pinto, D., et al. Nature 466(7304):368-372(2010) 3. Kim, Y., et al. J. Microbiol. Biotechnol. 18(3):600-610(2008) 4. Lee, T.S., et al. Ann. N. Y. Acad. Sci. 1091, 218-224 (2006) : 5. Shim, H., et al. Biotechnol. Lett. 28(24):2057-2063(2006)
USBio References
No references available
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