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035828-FITC Rabbit Anti-G6PC, ID (G6PC, G6PT, Glucose-6-phosphatase, Glucose-6-phosphatase alpha) (FITC)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P35575
Conjugate
FITC
Isotype
IgG
Grade
Affinity Purified
Applications
FLISA IHC WB
Crossreactivity
Hu
Gene ID
2538
Gene #
G6PC
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
G6PC, G6PT, Glucose-6-phosphatase, Glucose-6-phosphatase alpha

Glucose-6-phosphatase is an integral membrane protein of the endoplasmic reticulum that catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate. It is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Defects in the enzyme cause glycogen storage disease type I.

Applications
Suitable for use in FLISA, Western Blot and Immunohistochemisty. Other potential application, though not tested is Direct Flow Cytometry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Formalin-fixed, paraffin-embedded sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within aa123-149 in the central region of human G6PC.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human G6PC.
References
1. Tu, E., et al. Hum. Pathol. 41(3):392-400(2010). 2. Samuel, V.T., et al. Proc. Natl. Acad. Sci. U.S.A. 106(29):12121-12126(2009). 3. Hu, C., et al. Diabetologia 52(3):451-456(2009)
USBio References
No references available
Conjugates
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