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036035 Rabbit Anti-GIF, ID (GIF, IFMH, Gastric intrinsic factor, Intrinsic factor)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P27352
Isotype
IgG
Grade
Affinity Purified
Applications
E FC IC IF IHC WB
Crossreactivity
Hu
Accession #
NP_005133.2; P27352
Gene ID
2694
Gene #
GIF
Shipping Temp
Blue Ice
Storage Temp
-20°C
GIF, IFMH, Gastric intrinsic factor, Intrinsic factor

This gene is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia.

Applications
Suitable for use in ELISA, Indirect Flow Cytometry, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested.
Recommended Dilutions
Indirect Flow Cytometry: 1:10-1:50 Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:10-1:50 Immunofluorescence (IC): 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 189-218 in the central region of human GIF, UniProt Accession #NP_005133.2; P27352.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human GIF.
References
1. Levine, A.J., et al. Cancer Epidemiol. Biomarkers Prev. 19(7):1812-1821(2010) 2. Andersen, C.B., et al. Nature 464(7287):445-448(2010) 3. Ament, A.E., et al. Br. J. Haematol. 144(4):622-624(2009) 4. Remacha, A.F., et al. Ann. Hematol. 87(7):599-600(2008) 5. Mathews, F.S., et al. Proc. Natl. Acad. Sci. U.S.A. 104(44):17311-17316(2007)
USBio References
No references available
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