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036634 Rabbit Anti-HMGA1, CT (HMGA1, HMGIY, High mobility group protein HMG-I/HMG-Y, High mobility group AT-hook protein 1, High mobility group protein R)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P17096
Isotype
IgG
Grade
Purified
Applications
E FC WB
Crossreactivity
Hu Mo
Gene ID
3159
Gene #
HMGA1
Shipping Temp
Blue Ice
Storage Temp
-20°C
HMGA1, HMGIY, High mobility group protein HMG-I/HMG-Y, High mobility group AT-hook protein 1, High mobility group protein R

HMGA1 encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus.

Applications
Suitable for use in Western Blot, Indirect Flow Cytometry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-100 Indirect Flow Cytometry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
HMGA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71~101 amino acids from the C-terminal region of human HMGA1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Specificity
Recognizes human HMGA1. Species Crossreactivity: mouse
References
Mu,G., et.al., Hum. Pathol. 41 (4), 493-502 (2010)|Kim,J.J., et.al., J. Hum. Genet. 55 (1), 27-31 (2010)
USBio References
No references available
Conjugates
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