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037615 Rabbit Anti-KRT3, ID (KRT3, Keratin, type II cytoskeletal 3, 65kD cytokeratin, Cytokeratin-3, Keratin-3, Type-II keratin Kb3)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P12035
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_476429.2
Gene ID
3850
Gene #
KRT3
Shipping Temp
Blue Ice
Storage Temp
-20°C
KRT3, Keratin, type II cytoskeletal 3, 65kD cytokeratin, Cytokeratin-3, Keratin-3, Type-II keratin Kb3

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the corneal epithelium with family member KRT12 and mutations in these genes have been associated with Meesmann's Corneal Dystrophy. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 414-441 in the central region of human KRT3.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human KRT3.
References
1. Szaflik, J.P., et al. Mol. Vis. 14, 1713-1718 (2008) 2. Schweizer, J., et al. J. Cell Biol. 174(2):169-174(2006) 3. Chen, Y.T., et al. Cornea 24(8):928-932(2005) 4. Rogers, M.A., et al. J. Invest. Dermatol. 124(3):536-544(2005) 5. Irvine, A.D., et al. Nat. Genet. 16(2):184-187(1997)
USBio References
No references available
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