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043945 Rabbit Anti-YBX1, ID (YBX1, NSEP1, YB1, Nuclease-sensitive element-binding protein 1, CCAAT-binding transcription factor I subunit A, DNA-binding protein B, Enhancer factor I subunit A, Y-box transcription factor, Y-box-binding protein 1)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P67809
Isotype
IgG
Grade
Affinity Purified
Applications
E IF WB
Crossreactivity
Hu
Accession #
NP_004550.2
Gene ID
4904
Gene #
YBX1
Shipping Temp
Blue Ice
Storage Temp
-20°C
YBX1, NSEP1, YB1, Nuclease-sensitive element-binding protein 1, CCAAT-binding transcription factor I subunit A, DNA-binding protein B, Enhancer factor I subunit A, Y-box transcription factor, Y-box-binding protein 1

Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as HLA class II genes. Regulates the transcription of numerous genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability.

Applications
Suitable for use in Western Blot, Immunofluorescence and ELISA. Other applications have not been tested.
Recommended Dilution
Immunofluorescence: 1:10-1:50 Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 149-178 in the central region of human YBX1, UniProt Accession #P67809. Species sequence homology: bovine, mouse, rabbit, rat
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human YBX1.
References
Yu, Y.N., et al. Int. J. Oncol. 37(2):483-492(2010) Lovett, D.H., et al. Biochem. Biophys. Res. Commun. 398(3):482-488(2010) Takahashi, M., et al. Cancer Sci. 101(6):1367-1373(2010) Cobbold, L.C., et al. Oncogene 29(19):2884-2891(2010) To, K., et al. Cancer Res. 70(7):2840-2851(2010)
USBio References
No references available
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