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045084 Nigericin Sodium Salt CAS: 28643-80-3

Specifications
References
CAS Number
28643-80-3
Grade
Highly Purified
Molecular Formula
C40H67O11•Na
Molecular Weight
746.9
EU Commodity Code
38220090
UN DOT Shipping
UN3462 PGIII
Shipping Temp
Blue Ice
Storage Temp
-20°C
Antibiotic K 178; Helexin C; Polyetherin A; BRN 1696755; Azalomycin M; Antibiotic X-464

Antibiotic [1]. High affinity ionophore for monovalent cations such as H+, K+, Na+, Pb2+ [2, 3, 4, 7, 10]. Shows antibacterial (Gram-positive), antifungal, antitumor and antiviral activity [3, 6]. Disrupts membrane potential of mitochondria [2]. NLRP3/NALP3 activator. Signals through pannexin-1 to induce caspase-1 maturation and IL-1β processing and release [5, 8, 11, 12]. Autophagy modulator [13]

Source
Isolated from Streptomyces hygroscopicus
CAS No
28643-80-3
Molecular Formula
C40H67O11•Na
Molecular Weight
74.94
Purity (HPLC)
≥98%
Appearance
White to off-white powder
Solubility
Soluble in methanol, ethanol, dichlormethane or ethyl acetate. Insoluble in water.
Storage and Stability
Store at -20°C in the dark. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Toxicity and Hazards
 All products should be handled by qualified personnel only, trained in laboratory procedures.
References
1. Nigericin, a new crystalline antibiotic from an unidentified streptomyces: R. L. Harned, et al.; Antibiot. Chemother. 1, 594 (1951).  2. Potassium Ion-dependent hydrolysis of adenosine triphosphate induced by nigericin in mitochondria: S. Estrada-O, et al.; J. Biol. Chem. 242, 2925 (1967).  3. Teratogenic effects of nigericin, a carboxylic ionophore: G.C. Vedel-Macrander & R.D. Hood; Teratology 33, 47 (1986). 4. K+/H+-antiporter nigericin arrests DNA synthesis in Ehrlich ascites carcinoma cells: L.B. Margolis, et al.; PNAS 86, 6626 (1989). 5. IL-1 beta maturation: evidence that mature cytokine formation can be induced specifically by nigericin: D. Perregaux, et al.; J. Immunol. 149, 1294 (1992). 6. Antitumor effect of k+/h+-antiporter nigericin on human lung-carcinoma grown in in-vivo-like histocultures: B. Baibakov, et al.; Int. J. Oncol. 3, 1127 (1993). 7. Nigericin-induced Na+/H+ and K+/H+ exchange in synaptosomes: effect on [3H]GABA release: R. Rodríguez & M. Sitges; Neurochem. Res. 21, 889 (1996). 8. Activation of interleukin-1beta-converting enzyme by nigericin is independent of apoptosis: N. Watanabe, et al.; Cytokine 10, 645 (1998). 9. Nigericin inhibits insulin-stimulated glucose transport in 3T3-L1 adipocytes: C.Y. Chu, et al.; J. Cell Biochem. 85, 83 (2002). 10. The ionophore nigericin transports Pb2+ with high activity and selectivity: a comparison to monensin and ionomycin: S.A. Hamidinia, et al.; Biochemistry 43, 15956 (2004). 11. Cryopyrin activates the inflammasome in response to toxins and ATP: S. Mariathasan, et al.; Nature 440, 228 (2006). 12. Pannexin-1 couples to maitotoxin- and nigericin-induced interleukin-1beta release through a dye uptake-independent pathway: P. Pelegrin & A. Surprenant; J. Biol. Chem. 282, 2386 (2007).|13. Nigericin-induced impairment of autophagic flux in neuronal cells is inhibited by overexpression of Bak: J. Lim, et al.; J. Biol. Chem. 287, 23271 (2012)
USBio References
No references available
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