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045124 OX40L, Multimeric, Recombinant, Mouse (ACRP30headless:OX40L, ACRP30headless:CD252, ACRP30headless:CD134L) CAS:

Specifications
References
Grade
Highly Purified
Swiss Prot
P43488
Specificity
Binds to human and mouse OX40.
Molecular Weight
40
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C
ACRP30headless:OX40L, ACRP30headless:CD252, ACRP30headless:CD134L

OX40L is a membrane-expressed cytokine that belongs to the TNF ligand family. It acts as co-stimulator through interaction with its ligand OX40 on T cells, stimulating T cell activation, proliferation and cytokine production. OX40L is expressed on antigen presenting cells including B cells, dendritic cells, mast cells and endothelium.

This hexameric protein is a high activity construct in which two trimeric OX40L ligands are artificially linked via the collagen domain of ACRP30 and therefore mimics the membrane-bound form of the protein. This construct very effectively activates T cell proliferation.
Biological Activity
Activates T cell proliferation.
Sequence
Each monomer in this construct consists of the extracellular domain of mouse OX40L (aa 52-199) fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
HEK 293 cells
Concentration
~0.1mg/ml (after reconstitution)
Form
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS. Reconstitute with 100ul sterile dH2O.
Specificity
Binds to human and mouse OX40.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Endothelial cell co-stimulation through OX40 augments and prolongs T cell cytokine synthesis by stabilization of cytokine mRNA: J. Mestas, et al.; Int. Immun. 17, 737 (2005).|
USBio References
No references available
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