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045374 Trichostatin A (TSA, A300, R-(E,E)-7-(4-(dimethylamino)phenyl)-N-hydroxy- 4,6-dimethyl-7-oxo-2,4-heptadienamide) CAS: 58880-19-6

Specifications
References
CAS Number
58880-19-6
Grade
Highly Purified
Molecular Formula
C17H22N2O3
Molecular Weight
302.4
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
-20°C
TSA, A300, R-(E,E)-7-(4-(dimethylamino)phenyl)-N-hydroxy- 4,6-dimethyl-7-oxo-2,4-heptadienamide

Antibiotic. Antifungal. Antiprotozoal. Potent and reversible, cell permeable inhibitor of histone deacetylase (HDAC). Immunosuppressive. Anticancer compound. Antifibrogenic. Apoptosis inducer. Induces cell growth arrest at both G1 and G2/M phases. Enhances the efficacy of anticancer agents that target DNA. Inactivates mitotic spindle checkpoint. Smooth muscle cell proliferation inhibitor. Telomerase inhibitor. Downregulates DNA methyltransferase DNMT1 and affects DNA methylation. Anti-inflammatory. Inhibits osteoclastogenesis and bone resorption.

Synonyms
TSA; A300; R-(E,E)-7-(4-(dimethylamino)phenyl)-N-hydroxy- 4,6-dimethyl-7-oxo-2,4-heptadienamide
Source
Isolated from Streptomyces hygroscopicus
CAS Number
58880-19-6
Molecular Formula
C17H22N2O3
Molecular Weight
302.4
Solubility
DMSO, ethanol, methanol, dimethyl formamide, acetonitrile or chloroform. Insoluble in water or hexane.
Appearance
Supplied as an off-white solid.
Purity
≥95% (HPLC)
Storage and Stability
Store at -20°C. After reconstitution, prepare aliquots and store at -20°C. Stable for 1 year after receipt when stored at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Source
Streptomyces hygroscopicus
Purity
≥95% (HPLC)
Form
Supplied as an off-white solid.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
●A new antifungal antibiotic, trichostatin: N. Tsuji, et al.; J. Antibiot. (Tokyo) 29, 1 (1976)|●Screening for new antitrichomonal substances of microbial origin and antitrichomonal activity of trichostatin A: K. Otoguro, et al.; J. Antibiot. (Tokyo) 41, 461 (1988)|●Potent and specific inhibition of mammalian histone deacetylase both in vivo and in vitro by trichostatin A: M. Yoshida, et al.; J. Biol. Chem. 265, 17174 (1990)|●Selective inhibition of IL-2 gene expression by trichostatin A, a potent inhibitor of mammalian histone deacetylase: I. Takahashi, et al.; J. Antibiot. (Tokyo) 49, 453 (1996)|●Trichostatin A modulates expression of p21waf1/cip1, Bcl-xL, ID1, ID2, ID3, CRAB2, GATA-2, hsp86 and TFIID/TAFII31 mRNA in human lung adenocarcinoma cells: B. Eickhoff, et al.; Biol. Chem. 381, 107 (2000)|●Trichostatin A is a histone deacetylase inhibitor with potent antitumor activity against breast cancer in vivo: D.M. Vigushin, et al.; Clin. Cancer Res. 7, 971 (2001)|●Trichostatin A, a histone deacetylase inhibitor, suppresses collagen synthesis and prevents TGF-beta(1)-induced fibrogenesis in skin fibroblasts: K. Rombouts, et al.; Exp. Cell Res. 278, 184 (2002)|●The histone deacetylase inhibitor Trichostatin A modulates CD4+ T cell responses: J.M. Moreira, et al.; BMC Cancer 3, 30 (2003)|●Mechanism of histone deacetylase inhibitor Trichostatin A induced apoptosis in human osteosarcoma cells: M.S. Roh, et al.; Apoptosis 9, 583 (2004)|●Cell growth inhibition and gene expression induced by the histone deacetylase inhibitor, trichostatin A, on human hepatoma cells: T. Chiba, et al.; Oncology 66, 481 (2004)|●A histone deacetylase inhibitor, trichostatin A, enhances radiosensitivity by abrogating G2/M arrest in human carcinoma cells: I.A. Kim, et al.; Cancer Res. Treat. 37, 122 (2005)|●Mitotic spindle checkpoint inactivation by trichostatin a defines a mechanism for increasing cancer cell killing by microtubule-disrupting agents: M. Dowling, et al.; Cancer Biol. Ther. 4, 197 (2005)|●Trichostatin A, an inhibitor of histone deacetylase, inhibits smooth muscle cell proliferation via induction of p21(WAF1): H. Okamoto, et al.; J. Atheroscler. Thromb. 13, 183 (2006)|●Induction of apoptosis and inhibition of telomerase activity by trichostatin A, a histone deacetylase inhibitor, in human leukemic U937 cells: H.J. Woo, et al.; Exp. Mol. Pathol. 82, 77 (2007)|●Trichostatin A down-regulate DNA methyltransferase 1 in Jurkat T cells: R. Januchowski, et al.; Cancer Lett. 246, 313 (2007)|●Trichostatin A causes p53 to switch oxidative-damaged colorectal cancer cells from cell cycle arrest into apoptosis: C. Habold, et al.; J. Cell Mol. Med. 12, 607 (2008)|●Anti-inflammatory effect of Trichostatin-A on murine bone marrow-derived macrophages: S.B. Han & J.K. Lee; Arch. Pharm. Res. 32, 613 (2009)|●Trichostatin A inhibits osteoclastogenesis and bone resorption by suppressing the induction of c-Fos by RANKL: H.N. Kim, et al.; Eur. J. Pharmacol. 623, 22 (2009)|●Trichostatin A inhibits expression of cathepsins in experimental osteoarthritis: W.P. Chen, et al.; Rheumatol. Int. 31, 1325 (2011)
USBio References
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