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045439 lldD, Recombinant, E. coli, aa1-396, His-Tag (L-lactate Dehydrogenase [Cytochrome], lctD, b3605, JW3580) CAS:

Specifications
References
Grade
Highly Purified
Molecular Weight
45.3
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

lldD, also known as L-lactate dehydrogenase, is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD).

Source
Recombinant corresponding to aa1-396 from E.coli lldD, fused to His-tag at N-terminus, expressed in E.coli.
Molecular Weight
~45.3kD (420aa) confirmed by MALDI-TOF
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMIISAA SDYRAAAQRI LPPFLFHYMD GGAYSEYTLR RNVEDLSEVA LRQRILKNMS DLSLETTLFN EKLSMPVALA PVGLCGMYAR RGEVQAAKAA DAHGIPFTLS TVSVCPIEEV APAIKRPMWF QLYVLRDRGF MRNALERAKA AGCSTLVFTV DMPTPGARYR DAHSGMSGPN AAMRRYLQAV THPQWAWDVG LNGRPHDLGN ISAYLGKPTG LEDYIGWLGN NFDPSISWKD LEWIRDFWDG PMVIKGILDP EDARDAVRFG ADGIVVSNHG GRQLDGVLSS ARALPAIADA VKGDIAILAD SGIRNGLDVV RMIALGADTV LLGRAFLYAL ATAGQAGVAN LLNLIEKEMK VAMTLTGAKS ISEITQDSLV QGLGKELPAA LAPMAKGNAA
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
~90% (SDS-PAGE)
Concentration
~1mg/ml
Form
Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 10% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Ogura Y., et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106:17939-17944.
USBio References
No references available
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