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045449 NXT2, Recombinant, Human, aa1-197, His-Tag (NTF2-related Export Protein 2, Protein p15-2, BM-025, DC9) CAS:

Specifications
References
Grade
Highly Purified
Molecular Weight
25.3
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

NXT2 (nuclear transport factor 2-like export factor 2) is a member of NXT family proteins that are generally involved in exporting nuclear RNA in eukaryotic cells. This protein is regulator of protein export for NES-containing proteins. Also, it associates with NXF1, NXF2, NXF3 and NXF5 and plays a role in mRNA nuclear export. NXT2 has a critical role in maintaining morphogenetic integrity of embryonic heart in vertebrate species.

Source
Recombinant corresponding to aa1-197 from human NXT2, fused to His-tag at N-terminus, expressed in E.coli.
Molecular Weight
~25.3kD (221aa) confirmed by MALDI-TOF
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMRKYRS HWSQGDREGY QRRSNYYEGP HTSHSSPADR TREEVVTPTL PEHTATRSQM ATSLDFKTYV DQACRAAEEF VNIYYETMDK RRRALTRLYL DKATLIWNGN AVSGLDALNN FFDTLPSSEF QVNMLDCQPV HEQATQSQTT VLVVTSGTVK FDGNKQHFFN QNFLLTAQST PNNTVWKIAS DCFRFQDWSS S
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
≥95% (SDS-PAGE)
Concentration
~1mg/ml
Form
Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 2mM DTT, 200mM sodium chloride, 40% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Huang H, et al. (2005) BMC Dev Biol. 24;5:7. 2. Herold A, et al. (2000) Mol Cell Biol. 20(23):8996-9008.
USBio References
No references available
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