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045459 RPL8, Recombinant, Human, aa1-257, His-Tag (60S Ribosomal Protein L8) CAS:

Specifications
References
Grade
Purified
Molecular Weight
30.2
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

RPL8 (60S ribosomal protein L8) belongs to the ribosomal protein L2P family. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm and exists as a component of the 60S subunit where it is thought to play a role in aminoacyl-tRNA binding, specifically at the ribosomal subunit interface.

Recombinant protein corresponding to aa1-257 from human RPL8, fused to His-tag at N-terminus, expressed in E. coli.
Molecular Weight
~30.2kD (277aa) confirmed by MALDI-TOF
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH DPGRGAPLAK VVFRDPYRFK KRTELFIAAE GIHTGQFVYC GKKAQLNIGN VLPVGTMPEG TIVCCLEEKP GDRGKLARAS GNYATVISHN PETKKTRVKL PSGSKKVISS ANRAVVGVVA GGGRIDKPIL KAGRAYHKYK AKRNCWPRVR GVAMNPVEHP FGGGNHQHIG KPSTIRRDAP AGRKVGLIAA RRTGRLRGTK TVQEKEN
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
≥90% (SDS-PAGE)
Concentration
~0.1mg/ml
Form
Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 2mM DTT, 300mM sodium chloride, 2mM EDTA, 50% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Kenmochi, N., et al. (1998) Genome Res. 8: 509-523. 2. Hanes J, et al. (1994) Biochem Biophys Res Commun 197 (3): 1223-8.
USBio References
No references available
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