LAMA5

Clone Type

Monoclonal

Host

Mouse

Source

Human

Isotype

IgG1,k

Clone Number

2F7

Grade

Affinity Purified

Applications

E WB

Crossreactivity

Accession #

BC003355, AAH03355.1

Shipping Temp

Blue Ice

Storage Temp

-20°C

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Applications

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution

Optimal dilutions to be determined by the researcher.

AA Sequence

MGVSLRDKKVHWVYQLGEAGPAVLSIDEDIGEQFAAVSLDRTLQFGHMSVTVERQMIQETKGDTVAPGAEGLLNLRPDDFVFYVGGYPSTFTPPPLLRFP

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Partial recombinant corresponding to aa1-100 from human LAMA5 (AAH03355.1) with GST tag. MW of the GST tag alone is 26kD.

Form

Supplied as a liquid in PBS, pH 7.2.

Purity

Purified by Protein A affinity chromatography.

Specificity

Recognizes human LAMA5.

References

1. Genome-wide Runx2 occupancy in prostate cancer cells suggests a role in regulating secretion. Little GH, Noushmehr H, Baniwal SK, Berman BP, Coetzee GA, Frenkel B.Nucleic Acids Res. 2011 Dec 19. 2. Melanoma cells produce multiple laminin isoforms and strongly migrate on ?5 laminin(s) via several integrin receptors. Oikawa Y, Hansson J, Sasaki T, Rousselle P, Domogatskaya A, Rodin S, Tryggvason K, Patarroyo M.Exp Cell Res. 2010 Dec 31. [Epub ahead of print] 3. Proteomics Analysis of Nasopharyngeal Carcinoma Cell Secretome Using a Hollow Fiber Culture System and Mass Spectrometry. Wu HY, Chang YH, Chang YC, Liao PC.J Proteome Res. 2009 Jan;8(1):380-9. 4. Proteomic analysis of platelet a-granules using mass spectrometry. Maynard DM, Heijnen HF, Horne MK, White JG, Gahl WA.J Thromb Haemost. 2007 Sep;5(9):1945-55.

USBio References

No references available

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- Coupon Code

128991 Mouse Anti-LAMA5 (Laminin Subunit alpha-5, Laminin-10 Subunit alpha, Laminin-11 Subunit alpha, Laminin-15 Subunit alpha, KIAA0533, KIAA1907)

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Source

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Grade

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Crossreactivity

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References

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