CABIN1

Clone Type

Monoclonal

Host

Rabbit

Source

Human

Swiss Prot

Q9Y6J0

Isotype

IgG

Clone Number

13G22(D2B9F)

Grade

Purified

Applications

IP WB

Crossreactivity

Gene ID

23523

Shipping Temp

Blue Ice

Storage Temp

-20°C

Calcineurin binding protein CABIN1 was originally identified as an inhibitor of the calcium-dependent serine/threonine phosphatase, calcineurin. CABIN1 inhibits calcineurin signaling in T cells, regulating T cell receptor (TCR) signaling, transcription, and apoptosis (1-4). CABIN1 represses myocyte enhancer factor 2 (MEF2)-mediated transcription by recruiting chromatin remodeling enzymes (5), and also negatively regulates the activity of the tumor suppressor p53 (6). In response to genotoxic stress, CABIN1 is degraded and releases its inhibition of p53, allowing p53 to elicit cellular stress responses (7). CABIN1 is also involved in regulation of chromatin structure as part of the highly conserved HIRA/UBN1/CABIN1/ASF1A (HUCA) histone chaperone complex (8,9).

Applications

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution

Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Synthetic peptide corresponding to residues near the C-terminal domain of human CABIN1. Species Sequence Homology: monkey

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Purity

Purified.

Specificity

Recognizes endogenous levels of total human CABIN1.

References

1. Sun, L. et al. (1998) Immunity 8, 703-11. 2. Youn, H.D. and Liu, J.O. (2000) Immunity 13, 85-94. 3. Liu, W. et al. (2001) Eur J Immunol 31, 1757-64. 4. Kim, M.J. et al. (2002) Proc Natl Acad Sci U S A 99, 9870-5. 5. Jang, H. et al. (2007) J Biol Chem 282, 11172-9. 6. Jang, H. et al. (2009) Nat Struct Mol Biol 16, 910-5. 7. Choi, S.Y. et al. (2013) Nucleic Acids Res 41, 2180-2190. 8. Rai, T.S. et al. (2011) Mol Cell Biol 31, 4107-18. 9. Tang, Y. et al. (2012) Biochemistry 51, 2366-77.

USBio References

No references available

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137418 Rabbit Anti-CABIN1 (Calcineurin Binding Protein CABIN1)

Clone Type

Host

Source

Swiss Prot

Isotype

Clone Number

Grade

Applications

Crossreactivity

Gene ID

Shipping Temp

Storage Temp

References

USBio References

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