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138692 MAPKAPK3, Recombinant, Human, aa1-382, His-Tag (MAP Kinase-activated Protein Kinase 3, 3PK, MAPKAP-K3, MAPKAP3, MAPKAPK-3, MK-3) CAS:

Specifications
References
Grade
Highly Purified
Molecular Weight
45.4
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

MAP kinase-activated protein kinase 3, also known as MAPKAPK3, is involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript.

Source
Recombinant protein corresponding to aa1-382 from human MAPKAPK3 at N-terminal, fused to His-tag expressed in E.coli.
Molecular Weight
~45.4kD (405aa)
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMDGETAE EQGGPVPPPV APGGPGLGGA PGGRREPKKY AVTDDYQLSK QVLGLGVNGK VLECFHRRTG QKCALKLLYD SPKARQEVDH HWQASGGPHI VCILDVYENM HHGKRCLLII MECMEGGELF SRIQERGDQA FTEREAAEIM RDIGTAIQFL HSHNIAHRDV KPENLLYTSK EKDAVLKLTD FGFAKETTQN ALQTPCYTPY YVAPEVLGPE KYDKSCDMWS LGVIMYILLC GFPPFYSNTG QAISPGMKRR IRLGQYGFPN PEWSEVSEDA KQLIRLLLKT DPTERLTITQ FMNHPWINQS MVVPQTPLHT ARVLQEDKDH WDEVKEEMTS ALATMRVDYD QVKIKDLKTS NNRLLNKRRK KQAGSSSASQ GCNNQ
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
≥95% (SDS-PAGE)
Concentration
~1mg/ml
Form
Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.2M sodium chloride, 1mM DTT, 20% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Voncken J.W., et al. (2005) J. Biol. Chem. 280:5178-5187 2. Mendoza H., et al. (2008) Biochem. J. 409:711-722.
USBio References
No references available
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