Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
ELISA: 1:100-1:200 Western Blot: 1:50-1:400 Immunohistochemistry (frozen): 1:50-1:500 Immunohistochemistry (Paraffin): 1:10-1:100 Immunocytochemistry: 1:50-1:500 Optimal dilutions to be determined by the researcher.
Amino Acid Sequence
MGHHHHHHSGSEF-GGIPTYGVG AGGFPGFGVG VGGIPGVAGV PGVGGVPGVG GVPGVGISPE AQAAAAAKAA KYGVGTPAAA AAKAAAKAAQ FGLVPGVGVA PGVGVAPGVG VAPGVGLAPG VGVAPGVGVA PGVGVAPGIG PGGVAAAAKS AAKVAAKAQL RAAAGLGAGI PGLGVGVGVP GLGVGAGVPG LGVGAGVPGF GAVPGALAAA KAAKYGAAVP GVLGGLGALG GVGIPGGVVG AGPAAAAAAA KAAAKAAQFG LVGAA
Positive Control (Supplied)
140104A: Suppled as a recombinant protein (aa392-645) in loading buffer (100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol). DAB: 10ul/well ECL: 5ul/well Store at -20°C. Stable for 6 months after receipt.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to aa392-645 with an N-Terminal His-Tag from human ELN, expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human ELN.
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