The gene for CCL19 is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. CCL19 may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
ELISA (Sandwich): 2-4ug/ml (assuming 100ul/well), will detect recombinant human MIP-3beta when used with biotinylated antigen affinity purified anti-human MIP-3beta (M1202-67) as the detection antibody at a concentration of ~0.5-1ug/ml. Western Blot: 1-2ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant human MIP-3beta is 2-4ng/lane, under reducing conditions. Immunohistochemistry: This antibody stained PBMC. The primary antibody was incubated at 5ug/ml for one hour at RT followed by a fluorescent labeled secondary antibody. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to highly purified human CCL19 expressed in E. coli.
Form
Supplied as a lyophilized powder. Reconstitute with 500ul sterile ddH2O.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human CCL19.
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