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149545 ZBP1, Recombinant, Human (Insulin-like Growth Factor 2 mRNA-binding Protein 1, IGF2 mRNA-binding Protein 1, IMP-1, Coding Region Determinant-binding Protein, CRD-BP, IGF-II mRNA-binding Protein 1, VICKZ Family Member 1, Zip Code-binding Protein 1, ZBP-1, Zipcode-binding Protein 1, IGF2BP1, CRDBP, VICKZ1) CAS:

Specifications
References
Grade
Highly Purified
Swiss Prot
Q9NZI8
Applications
E WB
EU Commodity Code
30021019
Shipping Temp
Dry Ice
Storage Temp
-70°C

As Gram-negative bacterial isolates producing plasmid-mediated IMP-1 metallo-beta-lactamase usually demonstrate resistance to various broad-spectrum beta-lactams, including cephamycins and carbapenems, transmission and proliferation of these microorganisms in clinical settings could become a clinical threat in the near future.

Source
Recombinant protein corresponding to human IMP-1, expressed in E. coli.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Source
E. coli
Purity
~90% (Nickel NTA Affinity Chromatography)
Concentration
As reported
Form
Supplied as a liquid in 20mM Tris-HCl, 150mM sodium chloride, 1mM EDTA, 5mM glutathione, 0.5mM GSSH, 10% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Yamaguchi Y. (2005) Probing the role of Asp-120(81) of metallo-beta-lactamase (IMP-1) by site-directed mutagenesis, kinetic studies, and X-ray crystallography. J Biol Chem. 280(21): 20824-32.2. Nielsen J. (1999) A family of insulin-like growth factor II mRNA-binding proteins represses translation in late development. Mol Cell Biol. 19(2): 1262-70.
USBio References
No references available
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