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149550 Tumor Necrosis Factor, Recombinant, Human (TNF, TNF-alpha, TNFA, TNF-a, Cachectin, DIF, Tumor Necrosis Factor Ligand Superfamily Member 2, TNFSF2) CAS:

Specifications
References
Grade
Highly Purified
Swiss Prot
P01375
Applications
E WB
EU Commodity Code
30021019
Shipping Temp
Dry Ice
Storage Temp
-70°C

TNF-alpha belongs to the tumor nexrosis factor family and is believed to play roles in antitumor activity, immune moedulation, infalmmation, anorexia, cachexia, septicshock, viral replication and ematopoiesis. TNF-alpha is currently being evaluated in treatment of certain cancers and AIDS related symptoms.

Source
Recombinant protein corresponding to human TNF-alpha, expressed in E. coli.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Source
E. coli
Purity
~90% (Nickel NTA Affinity Chromatography)
Concentration
As reported
Form
Supplied as a liquid in 20mM Tris-HCl, 150mM sodium chloride, 1mM EDTA, 5mM glutathione, 0.5mM GSSH, 10% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Ware, C (1998) Tumor necrosis factor-related ligands and receptors. The Handbook 3rd ed, 549. 2. Eskandari, MK (1990) Immunol Invest 19:69-79.
USBio References
No references available
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