All three isoforms of GRO are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils. Recombinant murine MIP-2 is a 7.8kD protein consisting of 73aa including the 'ELR' motif common to the CXC chemokine family that bind to CXCR1 or CXCR2.
Source
Recombinant protein corresponding to mouse MIP-2, expressed in E.coli.
Biological Activity
Determined by its ability to chemoattract total human neutrophils using a concentration range of 1-10ng/ml.
Endotoxin
<0.1ng/ug (1EU/ug)
AA Sequence
AVVASELRCQ CLKTLPRVDF KNIQSLSVTP PGPHCAQTEV IATLKGGQKV CLDPEAPLVQ KIIQKILNKG KAN
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity
~98% (SDS-PAGE and HPLC)
Form
Supplied as a lyophilized powder.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.