C-C Motif Chemokine 19, CKb11, EBI1 Ligand Chemokine, Elc, ELC, Epstein-Barr Virus-induced Molecule 1 Ligand Chemokine, Exodus-3, MIP3B, Scya19, Small-inducible Cytokine A19
MIP-3beta is a CC chemokine, expressed in the thymus, lymph nodes, and in activated bone marrow stromal cells that signals through the CCR7 receptor. MIP-3beta is a chemoattractant for T and B lymphocytes, and myeloid progenitor cells. Human MIP-3beta is active on murine cells. Recombinant Murine MIP-3beta is a 9.2kD protein containing 83 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.
Recombinant protein corresponding to mouse MIP-3 beta, expressed in E.coli. Accession Number: NP_036018.1
Biological Activity
Determined by its ability to chemoattract activated human T cells expressing the CCR7 receptor. The ED50 for this effect is 30-90ng/ml.
Amino Acid Sequence
GANDAEDCCL SVTQRPIPGN IVKAFRYLLN EDGCRVPAVV FTTLRGYQLC APPDQPWVDR IIRRLKKSSA KNKGNSTRRS PVS
Storage and Stability
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Form
Supplied as a lyophilized powder. Reconsitute in sterile ddH2O, 0.1% HSA or BSA to 0.1-1mg/ml. Do not vortex.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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