Human A33, also known as GPA33, is a 43kD type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM A-C. The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain.
Applications
Suitable for use in Flow Cytometry, Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 2.5ug/10e6 cells. Tested using TH-29 human colon carcinoma cell line Western Blot: 0.1ug/ml using recombinat human A33 Immunohistochemistry: 5-15ug/ml using immersion fixed paraffin-embedded sections of human colon Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Ile22-Val235 from human A33 expressed in NS0 cells.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography
Specificity
Recognizes human A33. Crossreacts with recombinant mouse A33 ~10%.
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