Human A33, also known as GPA33, is a 43kD type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM-A-C. The extracellular domain (ECD) of human A33 is 214aa in length and contains one V-type and one C2-type Ig-like domain. This ECD is 80%, 74% and 71% aa identical to canine, bovine and mouse A33 ECD, respectively. A33 is likely to be involved in cell-cell adhesion between epithelial cells.
Applications
Suitable for use in Flow Cytometry, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 2.5ug stains 10e6 cells from the HT-29 human colon carcinoma cell line. Western Blot: 1ug/ml Immunohistochemistry: 8-25ug/ml stains immersion fixed paraffin-embedded sections of human colon and human colon cancer tissue subjected to antigen retrieval, basic. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Ile22-Val235 from human A33 expressed in NS0 cells.
Form
Supplied as a lyophilized powder from PBS, pH 6.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography from culture supernatant.
Specificity
Recognizes human A33. Does not crossreact with recombinant mouse A33.
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