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A0004-01C Goat Anti-ABCB5 (ATP-binding cassette, sub-family B MDR/TAP, ABCB5alpha, ABCB5beta, EST422562, ATP-binding cassette, sub-family B, member 5N: P-glycoprotein ABCB5)

Specifications
References
Clone Type
Polyclonal
Host
Goat
Source
Human
Swiss Prot
Q2M3G0
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_848654.3
Shipping Temp
Blue Ice
Storage Temp
-20°C
ATP-binding cassette, sub-family B (MDR/TAP), member 5 ; ABCB5alpha; ABCB5beta; EST422562; ATP-binding cassette, sub-family B, member 5N: P-glycoprotein ABCB5

ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. A number of distinct forms of ABCB5 have been shown to be expressed in various tissue types, including, but not limited to, melanocytes, melanoma cells, testis, mammary tissue, and retinal pigmented epithelium. ABCB5 is also indicated to be a metastatic melanoma progression marker.

Applications
Suitable for use in ELISA, Western Blot. Other applications not tested.
Recommended Dilution
Peptide ELISA: <1:128,000 Western Blot: 0.03-0.1ug/ml. Detects a band at ~80kD in lystaes of A431. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-QTQHRNTSKKAQ, from the internal region of human ABCB5 (NP_848654.3).
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human ABCB5 at ~80kD.
References
ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma., Frank NY, Margaryan A, Huang Y, Schatton T, Waaga-Gasser AM, Gasser M, Sayegh MH, Sadee W, Frank MH., Cancer Res. 2005 May 15;65(10):4320-33.
USBio References
No references available
Conjugates
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