A Broth

Grade

Molecular Biology Grade

EU Commodity Code

38210000

Shipping Temp

Storage Temp

RT/4°C

Ammonium Sulfate, Potassium Phosphate Dibasic, Potassium Phosphate Monobasic, Sodium Citrate

A Broth is a minimal medium base used in the maintenance and propagation of E. coli.

Appearance

White to off-white, homogeneous, free flowing powder

Solubility (16.5g/L)

Colorless, clear, complete

6.8-7.2

Directions per Liter

Dissolve 16.5 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized, adjusting pH as necessary. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Storage

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Media Formulation

Components shown as

g/liter

Ammonium Sulfate	1
Potassium Phosphate Dibasic	10.5
Potassium Phosphate Mono	4.5
Sodium Citrate	0.5

Total g/L:

16.5g

References

1. Ausubel, F. M., et al., Current Protocols in Molecular Biology, John Wiley (1992). 2. Maniatis, T., et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press (1989).