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A0219-90G Goat Anti-ACAT1 (Acetyl-CoA Acetyltransferase, Mitochondrial, Acetoacetyl-CoA Thiolase, T2, ACAT, MAT)

Specifications
References
Clone Type
Polyclonal
Host
Goat
Source
Human
Swiss Prot
P24752
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Bo Hu Mo Rt
Accession #
NP_000010.1
Gene ID
38
Shipping Temp
Blue Ice
Storage Temp
-20°C

ACAT1 is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:8000 Western Blot: 0.01-0.03ug/ml, human liver lysates observed on ~45kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-KRVDFSKVPKLKT, from human ACAT1, at the internal region of the protein (NP_000010.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ACAT1. Species sequence homology: Bovine, mouse and rat.
References
1. Hongo S, Watanabe T, Arita S, Kanome T, Kageyama H, Shioda S, Miyazaki A, Leptin modulates ACAT1 expression and cholesterol efflux from human macrophages. American journal of physiology. Endocrinology and metabolism 2009 Aug 297(2):E474-82.
USBio References
No references available
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