N-acetylgalactosamine 4 sulfate 6-O-sulfotransferase (GalNAc4S-6ST) also known as B cell RAG-associated protein (BRAG), is a type II transmembrane Golgi protein that catalyzes the transfer of sulfate onto GalNAc (4 SO4) in chondroitin sulfate A and dermatan sulfate. The 469 aa rhGalNAc4S-6ST predicts a molecular mass of 55kD and migrates as two bands with molecular masses of approximately 68 and 73kD. The amino acid sequence of human GalNAc4S-6ST is 99%, 93%, 88% and 79% identical to that of chimpanzee, rat/mouse, canine, and chicken.