Alpha-1 acid glycoprotein (AGP), also known as orosomucoid-1, is an acute phase protein secreted by the liver. Serum AGP levels are elevated during inflammatory responses. AGP binds a wide range of molecules in the circulation. Human and mouse AGP share 43% amino acid sequence identity.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 2ug/ml using human liver tissue and human plasma Immunohistochemistry: 8-25ug/ml using immersion fixed paraffin-embedded sections of human liver Optimal dilutions to be determined by the researcher
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Human AGP from plasma
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein A affinity chromatography from hybridoma culture supernatant
Specificity
Recognizes human Alpha-1 acid glycoprotein
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