Suitable for use in mmunoelectrophoresis and Single and Double Radial Immunodiffusion. Other applications not tested.

Immunoelectrophoresis: 2ul serum or equivalent against 120ul antiserum Double Radial Immunodiffusion: Use a rosette arrangement with 10ul antiserum in 3mm diameter center well and 2ul amniotic fluid or hepatoma serum sample (neat and serially diluted) in 2mm diameter peripheral wells. Optimal dilutions to be determined by the researcher.

Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Supplied as a lyophilized powder from PBS, pH 7.2. Preservative free. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.

Serum

Recognizes human alpha-1 Orosomucoid. Does not react with any other protein component of human serum or plasma as tested in gel diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins of different species frequently share antigenic determinants. Species cross-reactivity of this antiserum has not been tested in detail.