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A0725-52 Adiponectin, Human BioAssay™ ELISA Kit, High Sensitivity

Specifications
References
Brand
BioAssay™
Kit Type
Sandwich ELISA
Tests
96
Sensitivity
0.47ng/ml/0.156ng/ml
Detection Method
Colorimetric
Sample Matrix
serum, plasma, tissue culture
EU Commodity Code
38220000
UN DOT Shipping
UN2796 PGII
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

This kit is a sandwich ELISA for the quantitative measurement of human adiponectin in serum, plasma, breast milk, urine, cerebrospinal fluid and tissue culture media. It is intended for in vitro and research use only.

Features
• The total assay time is less than three hours. • The kit measures total adiponectin. • Standards are recombinant adiponectin based. • Quality controls are human serum based. No animal sera are used. • Components of the kit are ready-to-use (with the exception of Dilution Buffer and Wash Solution).
Kit Components
A0725-52A: Microtiter Plate, 1x96 wells A0725-52B: Adiponectin(HRP), 1x13ml A0725-52C: High Control,1x50ul A0725-52D: Low Control, 1x50ul A0725-52E: Dilution Buffer (10X), 2x10ml A0725-52F: Wash Solution (5X), 1x100ml A0725-52G: Substrate (TMB) Solution, 1x13ml A0725-52H: Stop Solution (0.2 M H2SO4), 1x13ml A0725-52J: Standards 150ng/ml; 1x1ml A0725-52K: Standards 100ng/ml 1x1ml A0725-52L: Standards 50ng/ml 1x1ml A0725-5M: Standards 20ng/ml 1x1ml A0725-52N: Standards10ng/ml 1x1ml A0725-52P: Standards 5ng/ml 1x1ml A0725-52Q: Standards 2ng/ml 1x1ml A0725-52R: Standards 1ng/ml 1x1ml
Storage and Stability
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
References
1. Arismendi E., et al. 2014. PLOS One. 9(9):e107859. 2. Arismendi E., et al. Obes Surg. 2015 Jan 25. [Epub ahead of print].|Other References:1. Scherer, P.E., et al., J. Biol. Chem. 270: 26,746-26,749 (1995). 2. Maeda, K., et al., Biochem. Biophys. Res. Commun. 221: 286-289 (1996). 3. Arita, Y., et al., Biochem. Biophys. Res. Commun. 257: 79-83 (1999). 4. Das, K., et al., Biochem. Biophys. Res. Commun. 280: 1120-1129 (2001). 5. Wang, Y., et al., J. Biol. Chem. 277: 19,521-19,529 (2002). 6. Kondo, H., et al., Diabetes 51: 2325-2328 (2002). 7. Spranger, J., et al., Lancet 361: 226-228 (2003). 8. Pajvani, U.B., et al., J. Biol. Chem. 278: 9073-9085 (2003). 9. Yamauchi, T., et al., J. Biol. Chem. 278: 2461-2468 (2003). 10. Yamauchi, T., et al., Nature 423: 762-769 (2003). 11. Waki, H., et al., J. Biol. Chem. 278: 40,352-40,363 (2003). 12. Ishikawa, Y., et al., Histopathology 42: 43-52 (2003). 13. Ouchi, N., et al., J. Biol. Chem. 279: 1304-1309 (2004).
USBio References
1. Arismendi E., et al. 2014. The Systemic Inflammome of Severe Obesity before and after Bariatric Surgery. PLOS One. 9(9):e107859. 2. Arismendi E, et al. Airway Hyperresponsiveness to Mannitol in Obesity Before and After Bariatric Surgery. Obes Surg. 2015 Jan 25. [Epub ahead of print].
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